1a2n
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STRUCTURE OF THE C115A MUTANT OF MURA COMPLEXED WITH THE FLUORINATED ANALOG OF THE REACTION TETRAHEDRAL INTERMEDIATE
| Authors | Skarzynski, T. Kim, D.H. Lees, W.J. Walsh, C.T. Duncan, K. |
| Citation | Stereochemical course of enzymatic enolpyruvyl transfer and catalytic conformation of the active site revealed by the crystal structure of the fluorinated analogue of the reaction tetrahedral intermediate bound to the active site of the C115A mutant of MurA |
| Release date | 1998-04-29 |
| Exp. Method | X-RAY DIFFRACTION |
| Resolution | 2.8 Å |
| Classification | TRANSFERASE |
Sequence
Chain A (419 residues): Blast Uniprot EC 2.5.1.7MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNVPKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVF CAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGATIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGVERLGGGVYRVLPDRIETGTFLV AAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVGEDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGT GFITETVFENRFMHVPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRIYHIDRGYE RIEDKLRALGANIERVKGE
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Categories: A2 | PDB entry | X-RAY DIFFRACTION | TRANSFERASE | ESCHERICHIA COLI | 1998 | EC 2.5.1.7
