1ari
From PDBWiki
ASPARTATE AMINOTRANSFERASE, W140H MUTANT, MALEATE COMPLEX
| Authors | Vacca, R.A. Christen, P. Malashkevich, V.N. Jansonius, J.N. Sandmeier, E. |
| Citation | Substitution of apolar residues in the active site of aspartate aminotransferase by histidine. Effects on reaction and substrate specificity. PubMed |
| Release date | 2003-04-01 |
| Exp. Method | X-RAY DIFFRACTION |
| Resolution | 2.3 Å |
| Classification | TRANSFERASE (AMINOTRANSFERASE) |
Sequence
Chain A (396 residues): Blast Uniprot EC 2.6.1.1MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTPVLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQE LLFGKGSALINDKRARTAQTPGGTGALRVAADFLAKNTSVKRVWVSNPSHPNHKSVFNSAGLEVREYAYYDAENHTLDFD ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFARGLEEDAEGLRAFAAMHKELIV ASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMKAAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQR MRQLFVNTLQEKGANRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAIVAVLChain B (396 residues): Blast Uniprot EC 2.6.1.1
MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTPVLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQE LLFGKGSALINDKRARTAQTPGGTGALRVAADFLAKNTSVKRVWVSNPSHPNHKSVFNSAGLEVREYAYYDAENHTLDFD ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFARGLEEDAEGLRAFAAMHKELIV ASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMKAAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQR MRQLFVNTLQEKGANRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAIVAVL
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