1ejg

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Authors	Jelsch, C. Teeter, M.M. Lamzin, V. Pichon-Pesme, V. Blessing, R.H. Lecomte, C.
Citation	Accurate protein crystallography at ultra-high resolution: valence electron distribution in crambin. PubMed
Release date	2000-04-05
Exp. Method	X-RAY DIFFRACTION
Resolution	0.54 Å
Classification	PLANT PROTEIN

Sequence

Chain(s) A (48 residues): Blast Uniprot

TTCCPSIVARSNFNVCRLPGTPSEALICATYTGCIIIPGATCPGDYAN

User comments

Duplicated residues warning

Residues 22 and 25 are duplicated in this entry. They both appear twice with 2 different aminoacids. It seems that Crambin's sequence does have two alternative isoforms: PL and SI (corresponding alternative residues for positions 22 and 25) and that the crystal contains both forms. The way to annotate this is with atom alt locations, which is indeed what the authors have done. However alt locs are designed for specifying alternate locations of atoms of a residue. The use of alt locs in this entry is, as far as I know, not following a standard practice.

More importantly this breaks the assumption that a PDB entry corresponds to a single sequence. The SEQRES of course can only show one of the two forms.

See also other entries for the same protein that have the same issue: 1cbn, 1jxt, 1jxu, 1jxw, 1jxx, 1jxy

--Jmduarte 11:50, 24 July 2009 (EDT)

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