2hr0

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Structure of Complement C3b: Insights into Complement Activation and Regulation

Authors	Abdul Ajees, A. Gunasekaran, K. Volanakis, J.E. Narayana, S.V. Kotwal, G.J. Krishna Murthy, H.M.
Citation	The structure of complement C3b provides insights into complement activation and regulation. PubMed
Release date	2006-10-31
Exp. Method	X-RAY DIFFRACTION
Resolution	2.26 Å
Classification	IMMUNE SYSTEM

Sequence

Chain(s) B (915 residues): Blast Uniprot

SNLDEDIIAEENIVSRSEFPESWLWNVEDLKEPPKNGISTKLMNIFLKDSITTWEILAVSMSDKKGICVADPFEVTVMQD
FFIDLRLPYSVVRNEQVEIRAVLYNYRQNQELKVRVELLHNPAFCSLATTKRRHQQTVTIPPKSSLSVPYVIVPLKTGLQ
EVEVKAAVYHHFISDGVRKSLKVVPEGIRMNKTVAVRTLDPERLGREGVQKEDIPPADLSDQVPDTESETRILLQGTPVA
QMTEDAVDAERLKHLIVTPSGCGEQNMIGMTPTVIAVHYLDETEQWEKFGLEKRQGALELIKKGYTQQLAFRQPSSAFAA
FVKRAPSTWLTAYVVKVFSLAVNLIAIDSQVLCGAVKWLILEKQKPDGVFQEDAPVIHQEMIGGLRNNNEKDMALTAFVL
ISLQEAKDICEEQVNSLPGSITKAGDFLEANYMNLQRSYTVAIAGYALAQMGRLKGPLLNKFLTTAKDKNRWEDPGKQLY
NVEATSYALLALLQLKDFDFVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQKDAPDHQELNLDVSLQLPSRSSKIT
HRIHWESASLLRSEETKENEGFTVTAEGKGQGTLSVVTMYHAKAKDQLTCNKFDLKVTIKPAPETEKRPQDAKNTMILEI
CTRYRGDQDATMSILDISMMTGFAPDTDDLKQLANGVDRYISKYELDKAFSDRNTLIIYLDKVSHSEDDCLAFKVHQYFN
VELIQPGAVKVYAYYNLEESCTRFYHPEKEDGKLNKLCRDELCRCAEENCFIQKSDDKVTLEERLDKACEPGVDYVYKTR
LVKVQLSNDFDEYIMAIEQTIKSGSDEVQVGQQRTFISPIKCREALKLEEKKHYLMWGLSSDFWGEKPNLSYIIGKDTWV
EHWPEEDECQDEENQKQCQDLGAFTESMVVFGCPN

Chain(s) A (645 residues): Blast Uniprot

SPMYSIITPNILRLESEETMVLEAHDAQGDVPVTVTVHDFPGKKLVLSSEKTVLTPATNHMGNVTFTIPANREFKSEKGR
NKFVTVQATFGTQVVEKVVLVSLQSGYLFIQTDKTIYTPGSTVLYRIFTVNHKLLPVGRTVMVNIENPEGIPVKQDSLSS
QNQLGVLPLSWDIPELVNMGQWKIRAYYENSPQQVFSTEFEVKEYVLPSFEVIVEPTEKFYYIYNEKGLEVTITARFLYG
KKVEGTAFVIFGIQDGEQRISLPESLKRIPIEDGSGEVVLSRKVLLDGVQNLRAEDLVGKSLYVSATVILHSGSDMVQAE
RSGIPIVTSPYQIHFTKTPKYFKPGMPFDLMVFVTNPDGSPAYRVPVAVQGEDTVQSLTQGDGVAKLSINTHPSQKPLSI
TVRTKKQELSEAEQATRTMQALPYSTVGNSNNYLHLSVLRTELRPGETLNVNFLLRMDRAHEAKIRYYTYLIMNKGRLLK
AGRQVREPGQDLVVLPLSITTDFIPSFRLVAYYTLIGASGQREVVADSVWVDVKDSCVGSLVVKSGQSEDRQPVPGQQMT
LKIEGDHGARVVLVAVDKGVFVLNKKNKLTQSKIWDVVEKADIGCTPGSGKDYAGVFSDAGLTFTSSSGQQTAQRAELQC
PQPAA

User comments

Problems with structure

One should definitely read

to get some interesting information about this structure.

References:

Bert J C Janssen, Randy J Read, Axel T Br�nger, Piet Gros
Crystallography: crystallographic evidence for deviating C3b structure.
Nature: 2007, 448(7154);E1-2; discussion E2-3
[PubMed:17687277] [DOI] (I p)

Alexander Wlodawer, Wladek Minor, Zbigniew Dauter, Mariusz Jaskolski
Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures.
FEBS J.: 2008, 275(1);1-21
[PubMed:18034855] [DOI] (P p)

--Anonymous 13:39, 21 December 2008 (GMT)

Evidence of falsification

"The University of Alabama at Birmingham has issued a statement indicating that a former UAB researcher may have fabricated or falsified data about protein structures that are contained in the Protein Data Bank.

The university did not include a name of the researcher, but the Birmingham News this week reported that the scientist is H.M. Krishna Murthy and an independent review by BioInform of the PDB shows that Murthy is associated with all of the proteins that UAB said were falsified.

The newspaper said that a probe of Murthy's research has been underway since 2007.

"After a thorough examination of the available data, which included a re-analysis of each structure alleged to have been fabricated, the committee found a preponderance of evidence that structures 1bef, 1cmw, 1df9/2qid, 1g40, 1g44, 1l6l, 2ou1, 1rid, 1y8e, 2a01, and 2hr0 were more likely than not falsified and/or fabricated and recommended that they be removed from the public record," the university said in its statement this week."