pdbwiki

Outer membrane compositions enhance the rate of extracellular electron transport via cell-surface

Moss PPR-SMR protein PpPPR_64 influences the expression of a psaA-psaB-rps14 gene cluster and processing of the 23S-4.5S rRNA precursor in chloroplasts

Moss PPR-SMR protein PpPPR_64 is a pTAC2 homolog but is functionally distinct from pTAC2. PpPPR_64 is required for psaA gene expression and its function may have evolved in mosses. The pentatricopeptide repeat (PPR) proteins are key regulatory factors responsible for the control of plant organellar gene expression. A small subset of PPR proteins possess a C-terminal small MutS-related (SMR) domain and have diverse roles in plant organellar biogenesis. However, the function of PPR-SMR proteins is not fully understood.

Here, we report the function of PPR-SMR protein PpPPR_64 in the moss Physcomitrium patens. Phylogenetic analysis indicated that PpPPR_64 belongs to the same clade as the Arabidopsis PPR-SMR protein pTAC2. PpPPR_64 knockout (KO) mutants grew autotrophically but with reduced protonemata growth and the poor formation of photosystems’ antenna complexes.

Quantitative reverse transcription-polymerase chain reaction and RNA gel blot hybridization analyses revealed a significant reduction in transcript levels of the psaA-psaB-rps14 gene cluster but no alteration to transcript levels of most photosynthesis- and non-photosynthesis-related genes.

In addition, RNA processing of 23S-4.5S rRNA precursor was impaired in the PpPPR_64 KO mutants. This suggests that PpPPR_64 is specifically involved in the expression level of the psaA-psaB-rps14 gene and in processing of the 23S-4.5S rRNA precursor. Our results indicate that PpPPR_64 is functionally distinct from pTAC2 and is a novel PPR-SMR protein required for proper chloroplast biogenesis in P. patens.

Rat Cholesterol ELISA ELISA
E01A11128
Goat Cholesterol ELISA ELISA
E01A46041
Mouse Cholesterol ELISA ELISA
E01A19869

Outer membrane compositions enhance the rate of extracellular electron transport via cell-surface MtrC protein in Shewanella oneidensis MR-1

While cell membrane composition is critical for the function of membrane proteins, membrane modification has not been used to control the rate of extracellular electron transfer (EET) via the outer membrane protein complexes. Here, the rate of electron flow via the cell-surface redox protein, MtrC, was highly enhanced upon change in the outer membrane composition in Shewanella oneidensis MR-1.
The MR-1 strain was pre-cultured at 4 °C and 30 °C to initiate differentiation of membrane composition. The whole-cell difference electrochemical assay of wild-type and mutant strains lacking MtrC suggested that the rate of EET via MtrC increased approximately 18 times at 4 °C than 30 °C.
Circular dichroism spectroscopy showed that the molar exciton coupling coefficient for inter-heme interaction in MtrC increased in MR-1 at 4 °C than 30 °C. Results suggest that membrane modification may be a novel strategy for improving the efficiency of EET-based technologies.
Rat Cholesterol ELISA ELISA
BlueGene
Goat Cholesterol ELISA ELISA
BlueGene
Mouse Cholesterol ELISA ELISA
BlueGene

Leucine-rich alpha-2 glycoprotein (LRG): A novel acute phase protein expressed in stage 3 grade C periodontitis before and after periodontal therapy

 

Background: Leucine-rich alpha-2 glycoprotein (LRG) is a novel acute phase protein involved in inflammation-associated diseases and that considered to be induced by multiple proinflammatory cytokines.
This study aimed to investigate gingival crevicular fluid (GCF) and serum levels of LRG, interleukin (IL)-6 and tumor necrosis factor (TNF)-α in patients with stage 3 periodontitis before and after non-surgical periodontal treatment.
Methods: Twenty-five stage 3 periodontitis and twenty-five periodontally healthy individuals were enrolled in the study. Clinical periodontal measurements were recorded; periodontitis patients received non-surgical periodontal treatment, and GCF and serum samples were obtained at baseline and at 6 weeks after treatment. LRG, IL-6 and TNF-α were determined by ELISA.
Results: GCF and serum LRG, IL-6 and TNF-α were significantly higher in periodontitis group than healthy controls (P < 0.001). A significant decrease in GCF and serum LRG, IL-6 and TNF-α was detected after periodontal treatment compared to baseline values of periodontitis patients (P < 0.001).
Conclusion: Our findings revealed that LRG expression was increased in stage 3 periodontitis both locally and systemically, and non-surgical periodontal therapy was effective in reducing LRG levels in GCF and serum of these patients. This article is protected by copyright. All rights reserved.
Rat Cholesterol ELISA ELISA
E01A11128 BlueGene
Goat Cholesterol ELISA ELISA
E01A46041 BlueGene
Mouse Cholesterol ELISA ELISA
E01A19869 BlueGene
Human Cholesterol ELISA ELISA
E01A2368 BlueGene
Sheep Cholesterol ELISA ELISA
E01A98335 BlueGene

Aqueous Two-Phase-Assisted Precipitation of Proteins: A Platform for Isolation of Process-Related Impurities from Therapeutic Proteins

Aqueous two-phase systems (ATPS) have been widely and successfully used in the purification of various biological macromolecules such as proteins, nucleic acids, antibiotics, and cell components.
Interfacial precipitation of the product often results in lower recovery and selectivity of ATPS. Efficient resolubilization of the interfacial precipitate offers a way to improve the recovery as well as selectivity of ATPS systems.
pdbwiki
pdbwiki

Tyrosyl-DNA Phosphodiesterase 2 (Recombinant)

20-abx073819
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  • 10 ug
  • 1 mg
  • 2 µg

Human Tyrosyl- DNA phosphodiesterase 2, TDP2 ELISA KIT

ELI-16782h 96 Tests
EUR 988.8

Mouse Tyrosyl- DNA phosphodiesterase 2, Tdp2 ELISA KIT

ELI-51300m 96 Tests
EUR 1038

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

RPU57152-100ug 100ug
EUR 470.4

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

RPU57152-1mg 1mg
EUR 2184

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

RPU57152-50ug 50ug
EUR 385

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

RPC785Hu01 10ug
EUR 140

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

RPC785Mu01 10ug
EUR 132

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

4-RPC785Mu01
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  • 100 ug
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  • 200 ug
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  • 5 mg
Description: Recombinant Mouse Tyrosyl DNA Phosphodiesterase 1 expressed in: E.coli

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

RPC785Ra01 10ug
EUR 136

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

4-RPC785Ra01
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  • 100 ug
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  • 5 mg
Description: Recombinant Rat Tyrosyl DNA Phosphodiesterase 1 expressed in: E.coli

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

RPU50540-100ug 100ug
EUR 407

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

RPU50540-1mg 1mg
EUR 1801.8

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

RPU50540-50ug 50ug
EUR 326.7

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

RPU50584-100ug 100ug
EUR 419.1

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

RPU50584-1mg 1mg
EUR 1856.4

Recombinant Tyrosyl DNA Phosphodiesterase 1 (TDP1)

RPU50584-50ug 50ug
EUR 336.6

Bovine Tyrosyl- DNA phosphodiesterase 2, TDP2 ELISA KIT

ELI-17055b 96 Tests
EUR 1113.6

Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Antibody

20-abx126687
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  • 100 ul
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Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Antibody

20-abx211057
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  • 100 ul
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Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Antibody

20-abx211964
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  • 100 ul
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Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Antibody

abx126687-100l 100 µl
EUR 175

Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Antibody

abx126687-1ml 1 ml
EUR 387.5

Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Antibody

abx126687-200l 200 µl
EUR 275

Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Antibody

abx211057-100l 100 µl
EUR 350

Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Antibody

abx211057-50l 50 µl
EUR 250

Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Antibody

abx211964-100l 100 µl
EUR 350

Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Antibody

abx211964-50l 50 µl
EUR 250

Mouse Tyrosyl DNA Phosphodiesterase 1 (TDP1) Protein

20-abx650176
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  • 100 ug
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  • 200 ug
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Mouse Tyrosyl DNA Phosphodiesterase 1 (TDP1) Protein

abx650176-100g 100 µg
EUR 462.5

Mouse Tyrosyl DNA Phosphodiesterase 1 (TDP1) Protein

abx650176-10g 10 µg
EUR 200

Mouse Tyrosyl DNA Phosphodiesterase 1 (TDP1) Protein

abx650176-50g 50 µg
EUR 325

ELISA Kit for Tyrosyl DNA Phosphodiesterase 1 (TDP1)

SEC785Hu 96Т
EUR 700

Tyrosyl-DNA Phosphodiesterase 2 Human Recombinant

rAP-1095 Inquiry Ask for price

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

20-abx585197
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

EKU11505-48T 48T
EUR 555.66

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

EKU11505-5x96T 5x96T
EUR 3770.55

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

EKU11505-96T 96T
EUR 793.8

Mouse Tyrosyl- DNA phosphodiesterase 1, Tdp1 ELISA KIT

ELI-44743m 96 Tests
EUR 1038

Human Tyrosyl- DNA phosphodiesterase 1, TDP1 ELISA KIT

ELI-39932h 96 Tests
EUR 988.8

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

EKN52616-48T 48T
EUR 345.73

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

EKN52616-5x96T 5x96T
EUR 2346.03

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

EKN52616-96T 96T
EUR 493.9

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

SEC785Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

SEC785Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

SEC785Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

SEC785Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

4-SEC785Hu
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Tyrosyl DNA Phosphodiesterase 1 (TDP1) Polyclonal Antibody

CAU23870-100ul 100ul
EUR 241.9

Tyrosyl DNA Phosphodiesterase 1 (TDP1) Polyclonal Antibody

CAU23870-200ul 200ul
EUR 302.4

Human Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Protein

abx073819-20tests 20 tests
EUR 225

Human Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Protein

abx073819-50tests 50 tests
EUR 325

Human Tyrosyl-DNA Phosphodiesterase 2 (TDP2) Protein

abx073819-80tests 80 tests
EUR 6575

Tyrosyl-Dna Phosphodiesterase 1 (TDP1) Antibody

20-abx116391
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  • 150 ul
  • 50 ul

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

20-abx137321
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  • 100 ug
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  • 5 ug

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

20-abx007160
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  • 100 ul
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Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx029686-400ul 400 ul
EUR 627.6

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx029686-80l 80 µl
EUR 343.2

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

20-abx003676
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  • 100 ul
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Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx433352-200ul 200 ul
EUR 460.8

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx238572-100ug 100 ug
EUR 610.8

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

20-abx322676
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  • 100 ul
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Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

20-abx320393
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  • 100 ul
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Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx003676-100l 100 µl
EUR 400

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx003676-20l 20 µl
EUR 175

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx003676-50l 50 µl
EUR 275

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx007160-100g 100 µg
EUR 275

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx007160-10g 10 µg
EUR 175

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx007160-200g 200 µg
EUR 387.5

Tyrosyl-Dna Phosphodiesterase 1 (TDP1) Antibody

abx116391-100g 100 µg
EUR 612.5

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx029686-400l 400 µl
EUR 518.75

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx238572-100l 100 µl Ask for price

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx238572-50l 50 µl
EUR 350

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx137321-100tests 100 tests
EUR 225

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx433352-100g 100 µg
EUR 387.5

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx320393-100l 100 µl
EUR 350

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx320393-50l 50 µl
EUR 237.5

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx322676-100g 100 µg
EUR 350

Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

abx322676-50g 50 µg
EUR 237.5

Rat Tyrosyl-DNA Phosphodiesterase 2 (TDP2) ELISA Kit

abx551954-96tests 96 tests
EUR 687.5

Mouse Tyrosyl-DNA phosphodiesterase 2 (TDP2) ELISA Kit

abx551953-96tests 96 tests
EUR 687.5

Polyclonal Antibody to Tyrosyl DNA Phosphodiesterase 1 (TDP1)

PAC785Mu01 100ul
EUR 252

Tyrosyl-DNA Phosphodiesterase 1 Human Recombinant

rAP-1093 Inquiry Ask for price

Rat Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Protein

20-abx650870
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  • 100 ug
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  • 1 mg
  • 200 ug
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Rat Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Protein

abx650870-5mg 5 mg
EUR 337.5

Tyrosyl DNA Phosphodiesterase 1 (TDP1) Polyclonal Antibody (Mouse)

4-PAC785Mu01
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  • 100ul
  • 10ml
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Description: A Rabbit polyclonal antibody against Mouse Tyrosyl DNA Phosphodiesterase 1 (TDP1)

TDP2 (untagged)-Human tyrosyl-DNA phosphodiesterase 2 (TDP2)

SC324645 10 µg Ask for price

Human Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Protein

abx073385-10kDa1g 10 kDa; 1 g
EUR 325

Human Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Protein

abx073385-20kDa1g 20 kDa; 1 g
EUR 225

Human Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Protein

abx073385-5kDa1g 5 kDa; 1 g
EUR 3550

Tyrosyl DNA Phosphodiesterase 1 (TDP1) Magnetic Luminex Assay Kit

LKU607595-96T 96T
EUR 966

Human Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Antibody

31298-05111 150 ug
EUR 215

Rat Tyrosyl-DNA Phosphodiesterase 1 (TDP1) ELISA Kit

abx551949-96tests 96 tests
EUR 687.5

Tyrosyl DNA Phosphodiesterase 1 (TDP1) Polyclonal Antibody (Mouse), APC

4-PAC785Mu01-APC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Mouse Tyrosyl DNA Phosphodiesterase 1 (TDP1). This antibody is labeled with APC.

Tyrosyl DNA Phosphodiesterase 1 (TDP1) Polyclonal Antibody (Mouse), Cy3

4-PAC785Mu01-Cy3
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  • 100ul
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Description: A Rabbit polyclonal antibody against Mouse Tyrosyl DNA Phosphodiesterase 1 (TDP1). This antibody is labeled with Cy3.

Tyrosyl DNA Phosphodiesterase 1 (TDP1) Polyclonal Antibody (Mouse), HRP

4-PAC785Mu01-HRP
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  • 100ul
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Description: A Rabbit polyclonal antibody against Mouse Tyrosyl DNA Phosphodiesterase 1 (TDP1). This antibody is labeled with HRP.

Tyrosyl DNA Phosphodiesterase 1 (TDP1) Polyclonal Antibody (Mouse), PE

4-PAC785Mu01-PE
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  • 100ul
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Description: A Rabbit polyclonal antibody against Mouse Tyrosyl DNA Phosphodiesterase 1 (TDP1). This antibody is labeled with PE.

Tyrosyl-DNA Phosphodiesterase 1 Human Recombinant, Sf9

rAP-2062 Inquiry Ask for price

Human Tyrosyl-DNA Phosphodiesterase 1(TDP1)ELISA Kit

EKC35968-48T 48T
EUR 535.99

Human Tyrosyl-DNA Phosphodiesterase 1(TDP1)ELISA Kit

EKC35968-5x96T 5x96T
EUR 3637.08

Human Tyrosyl-DNA Phosphodiesterase 1(TDP1)ELISA Kit

EKC35968-96T 96T
EUR 765.7

Human Tyrosyl-DNA Phosphodiesterase 1(TDP1) Elisa Kit

EK713861 96 Wells
EUR 0.4

Human Tyrosyl-DNA Phosphodiesterase 1(TDP1)ELISA Kit

CSB-E17810h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Tyrosyl-DNA Phosphodiesterase 1 (TDP1) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Tyrosyl-DNA Phosphodiesterase 1(TDP1)ELISA Kit

1-CSB-E17810h
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  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Tyrosyl-DNA Phosphodiesterase 1(TDP1) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Tyrosyl-DNA phosphodiesterase 1 (TDP1) ELISA Kit

abx551948-96tests 96 tests
EUR 687.5

Tyrosyl DNA Phosphodiesterase 1 (TDP1) Polyclonal Antibody (Mouse), FITC

4-PAC785Mu01-FITC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Rabbit polyclonal antibody against Mouse Tyrosyl DNA Phosphodiesterase 1 (TDP1). This antibody is labeled with FITC.

TDP2 (GFP-tagged) - Human tyrosyl-DNA phosphodiesterase 2 (TDP2)

RG202015 10 µg Ask for price

TDP2 Tyrosyl-DNA Phosphodiesterase 2 Human Recombinant Protein

PROTO95551 Regular: 10ug
EUR 380.4
Description: TDP2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 385 amino acids (1-362) and having a molecular mass of 43.3kDa. ;TDP2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Tdp2 (untagged) - Mouse tyrosyl-DNA phosphodiesterase 2 (Tdp2), (10ug)

MC210026 10 µg Ask for price

Human Tyrosyl-DNA Phosphodiesterase 1, TDP1 GENLISA ELISA

KBH6368 1 x 96 wells
EUR 286
In this protocol, we describe a method for aqueous two-phase-assisted precipitation and resolubilization of the recombinant human Granulocyte Colony Stimulating Factor (GCSF) for its selective isolation from E. coli host cell proteins as well as nucleic acids.
This platform purification can be applied to other cytokines as well as most of the hydrophobic proteins that partition into the hydrophobic PEG-rich top phase. Recoveries of up to 100% of the product along with reduction of levels of E. coli host cell proteins (from 250-500 to 10-15 ppm) and of nucleic acids (from 15-20 to 5-15 ng/mL) were observed.
Rat Cholesterol ELISA ELISA
E01A11128
Goat Cholesterol ELISA ELISA
E01A46041
Mouse Cholesterol ELISA ELISA
E01A19869

Affinity Tags in Protein Purification and Peptide Enrichment: An Overview

The reversible interaction between an affinity ligand and a complementary receptor has been widely explored in purification systems for several biomolecules. The development of tailored affinity ligands highly specific toward particular target biomolecules is one of the options in affinity purification systems.
However, both genetic and chemical modifications in proteins and peptides widen the application of affinity ligand-tag receptors pairs toward universal capture and purification strategies.
In particular, this chapter will focus on two case studies highly relevant for biotechnology and biomedical areas, namely the affinity tags and receptors employed on the production of recombinant fusion proteins, and the chemical modification of phosphate groups on proteins and peptides and the subsequent specific capture and enrichment, a mandatory step before further proteomic analysis.
MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-48D Bioingentech
MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-96D Bioingentech
MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-48D Bioingentech
MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-96D Bioingentech
Leaf PCR Kit
11140007-1 Glycomatrix

Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins

Synthetic ligand affinity adsorbents offer an efficient means for purification of biopharmaceuticals. Single-isomer textile dye C.I. Reactive Blue and newer ligands developed by rational design and screening of chemical combinatorial libraries based on a triazine scaffold are routinely used for the capture and purification of these proteins from engineered recombinant expression systems.
Here, we describe methods for the purification of recombinant human serum albumin and related fusion proteins using synthetic ligand affinity adsorbents.
Paraffin Wax
P191410
Paraffin wax pellets
GRM10702-2KG
Paraffin wax pellets
GRM10702-500G
Paraffin wax Pellets
GRM10702W-2KG
Paraffin wax Pellets
GRM10702W-500G

Redox homeostasis and cell cycle activation mediate beta-cell mass expansion in aged, diabetes-prone mice under metabolic stress conditions: Role of thioredoxin-interacting protein (TXNIP)

 

Overnutrition contributes to insulin resistance, obesity and metabolic stress, initiating a loss of functional beta-cells and diabetes development. Whether these damaging effects are amplified in advanced age is barely investigated.
Therefore, New Zealand Obese (NZO) mice, a well-established model for the investigation of human obesity-associated type 2 diabetes, were fed a metabolically challenging diet with a high-fat, carbohydrate restricted period followed by a carbohydrate intervention in young as well as advanced age.
Interestingly, while young NZO mice developed massive hyperglycemia in response to carbohydrate feeding, leading to beta-cell dysfunction and cell death, aged counterparts compensated the increased insulin demand by persistent beta-cell function and beta-cell mass expansion.
MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-48D
MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-96D
PCR Mix
L5051100
Beta-cell loss in young NZO islets was linked to increased expression of thioredoxin-interacting protein (TXNIP), presumably initiating an apoptosis-signaling cascade via caspase-3 activation. In contrast, islets of aged NZOs exhibited a sustained redox balance without changes in TXNIP expression, associated with higher proliferative potential by cell cycle activation.
These findings support the relevance of a maintained proliferative potential and redox homeostasis for preserving islet functionality under metabolic stress, with the peculiarity that this adaptive response emerged with advanced age in diabetes-prone NZO mice.

GTPBP8 cloning plasmid

CSB-CL818697HU2-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the GTPBP8 gene.

Lenti ORF clone of Gtpbp8 (mGFP-tagged) - Mouse GTP-binding protein 8 (putative) (Gtpbp8), transcript variant 1

MR214079L4 10 µg Ask for price

Lenti ORF clone of Gtpbp8 (mGFP-tagged) - Mouse GTP-binding protein 8 (putative) (Gtpbp8), transcript variant 2

MR214608L4 10 µg Ask for price

GTPBP8

CSB-CL818697HU1 10 μg plasmid + 200μl Glycerol Ask for price

GTPBP8

CSB-CL818697HU2 10 μg plasmid + 200μl Glycerol Ask for price

Lenti ORF clone of Gtpbp8 (Myc-DDK-tagged) - Mouse GTP-binding protein 8 (putative) (Gtpbp8), transcript variant 1

MR214079L3 10 µg Ask for price

Lenti ORF clone of Gtpbp8 (Myc-DDK-tagged) - Mouse GTP-binding protein 8 (putative) (Gtpbp8), transcript variant 2

MR214608L3 10 µg Ask for price

Lenti ORF clone of Gtpbp5 (mGFP-tagged) - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063)

MR202797L4 10 µg Ask for price

Gtpbp5 (GFP-tagged) - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063)

MG202797 10 µg Ask for price

Gtpbp5 (untagged) - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063), (10ug)

MC217792 10 µg Ask for price

Lenti ORF clone of Gtpbp5 (Myc-DDK-tagged) - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063)

MR202797L3 10 µg Ask for price

Gtpbp5 (Myc-DDK-tagged) - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063)

MR202797 10 µg Ask for price

GTPBP8 siRNA

20-abx918914
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

GTPBP8 siRNA

20-abx918915
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

GTPBP8 siRNA

20-abx902374
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

GTPBP8 Antibody

E311513 200ul
EUR 275
Description: Available in various conjugation types.

GTPBP8 Rabbit pAb

E2516189 100ul
EUR 225
Description: Available in various conjugation types.

GTPBP8 Rabbit pAb

A16189-100ul 100 ul
EUR 369.6

GTPBP8 Rabbit pAb

A16189-200ul 200 ul
EUR 550.8

GTPBP8 Rabbit pAb

A16189-20ul 20 ul
EUR 219.6

GTPBP8 Rabbit pAb

A16189-50ul 50 ul
EUR 267.6

GTPBP8 Polyclonal Antibody

29768 100ul
EUR 439

GTPBP8 Polyclonal Antibody

29768-100ul 100ul
EUR 302.4

GTPBP8 Polyclonal Antibody

29768-50ul 50ul
EUR 224.4

GTPBP8 Polyclonal Antibody

E916189 100ul
EUR 225
Description: Available in various conjugation types.

GTPBP8 Polyclonal Antibody

E-AB-66717-120uL 120uL
EUR 320
Description: Unconjugated

GTPBP8 Polyclonal Antibody

E-AB-66717-200uL 200uL
EUR 530
Description: Unconjugated

GTPBP8 Polyclonal Antibody

E-AB-66717-60uL 60uL
EUR 200
Description: Unconjugated

GTPBP8 Polyclonal Antibody

E-AB-66717-each each Ask for price
Description: Unconjugated

Lenti ORF particles, Gtpbp5 (GFP-tagged) - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063), 200ul, >10^7 TU/mL

MR202797L4V 200 µl Ask for price

Gtpbp8 (untagged ORF) - Rat GTP-binding protein 8 (putative) (Gtpbp8), (10 ug)

RN210345 10 µg Ask for price

Rat GTPBP8 shRNA Plasmid

20-abx990090
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

Lenti ORF particles, Gtpbp5 (Myc-DDK-tagged) - Mouse GTP binding protein 5 (cDNA clone MGC:36295 IMAGE:4483063), 200ul, >10^7 TU/mL

MR202797L3V 200 µl Ask for price

Human GTPBP8 shRNA Plasmid

20-abx959177
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

Mouse GTPBP8 shRNA Plasmid

20-abx975375
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

GTPBP8 (untagged)-Human GTP-binding protein 8 (putative) (GTPBP8), transcript variant 1

SC304056 10 µg Ask for price

GTPBP8 (untagged)-Human GTP-binding protein 8 (putative) (GTPBP8), transcript variant 2

SC306032 10 µg Ask for price

GTPBP8 (untagged)-Human GTP-binding protein 8 (putative) (GTPBP8), transcript variant 1

SC320468 10 µg Ask for price

GTPBP8 Recombinant Protein (Rat)

RP203999 100 ug Ask for price

GTPBP8 Recombinant Protein (Mouse)

RP140480 100 ug Ask for price

GTPBP8 Recombinant Protein (Mouse)

RP140483 100 ug Ask for price

GTPBP8 Recombinant Protein (Human)

RP014239 100 ug Ask for price

GTPBP8 Recombinant Protein (Human)

RP014242 100 ug Ask for price

Gtpbp8 (Myc-DDK-tagged ORF) - Rat GTP-binding protein 8 (putative) (Gtpbp8), (10 ug)

RR210345 10 µg Ask for price

Gtpbp8 ORF Vector (Rat) (pORF)

ORF068001 1.0 ug DNA
EUR 607.2

Gtpbp8 (untagged) - Mouse GTP-binding protein 8 (putative) (Gtpbp8), transcript variant 1, (10ug)

MC210262 10 µg Ask for price

Gtpbp8 (untagged) - Mouse GTP-binding protein 8 (putative) (Gtpbp8), transcript variant 2, (10ug)

MC210263 10 µg Ask for price

GTPBP8 (GFP-tagged) - Human GTP-binding protein 8 (putative) (GTPBP8), transcript variant 1

RG205656 10 µg Ask for price

GTPBP8 (GFP-tagged) - Human GTP-binding protein 8 (putative) (GTPBP8), transcript variant 2

RG219767 10 µg Ask for price

GTPBP8 ORF Vector (Human) (pORF)

ORF004747 1.0 ug DNA
EUR 114

GTPBP8 ORF Vector (Human) (pORF)

ORF004748 1.0 ug DNA
EUR 114

Gtpbp8 ORF Vector (Mouse) (pORF)

ORF046828 1.0 ug DNA
EUR 607.2

Gtpbp8 ORF Vector (Mouse) (pORF)

ORF046829 1.0 ug DNA
EUR 607.2

GTPBP8 Polyclonal Conjugated Antibody

C29768 100ul
EUR 476.4

Lenti ORF clone of Human GTP-binding protein 8 (putative) (GTPBP8), transcript variant 1, mGFP tagged

RC205656L4 10 µg Ask for price

Lenti ORF clone of Human GTP-binding protein 8 (putative) (GTPBP8), transcript variant 2, mGFP tagged

RC219767L4 10 µg Ask for price

Human GTPBP8 Protein Lysate 20ug

IHUGTPBP8PLLY20UG each
EUR 213
Description: Human GTPBP8 Protein Lysate 20ug

Gtpbp8 (GFP-tagged) - Mouse GTP-binding protein 8 (putative) (Gtpbp8) transcript variant 1, (10ug)

MG214079 10 µg Ask for price

GTPBP8 (Myc-DDK-tagged)-Human GTP-binding protein 8 (putative) (GTPBP8), transcript variant 1

RC205656 10 µg Ask for price

Gtpbp8 (GFP-tagged) - Mouse GTP-binding protein 8 (putative) (Gtpbp8) transcript variant 2, (10ug)

MG214608 10 µg Ask for price

Gtpbp8 (Myc-DDK-tagged) - Mouse GTP-binding protein 8 (putative) (Gtpbp8), transcript variant 1

MR214079 10 µg Ask for price

Gtpbp8 (Myc-DDK-tagged) - Mouse GTP-binding protein 8 (putative) (Gtpbp8), transcript variant 2

MR214608 10 µg Ask for price

GTPBP8 (Myc-DDK-tagged)-Human GTP-binding protein 8 (putative) (GTPBP8), transcript variant 2

RC219767 10 µg Ask for price

OCOA11269-20UG - GTPBP8 Protein Lysate

OCOA11269-20UG 20ug
EUR 169

OCOA06483-20UG - GTPBP8 Protein Lysate

OCOA06483-20UG 20ug
EUR 169

GTPBP8 Over-expression Lysate Product

GWB-3E049E 0.1 mg Ask for price

Lenti ORF clone of Human GTP-binding protein 8 (putative) (GTPBP8), transcript variant 1, Myc-DDK-tagged

RC205656L3 10 µg Ask for price

Lenti ORF clone of Human GTP-binding protein 8 (putative) (GTPBP8), transcript variant 2, Myc-DDK-tagged

RC219767L3 10 µg Ask for price

ARP95882_P050 - Gtpbp8 Antibody Middle region

ARP95882_P050 100ul
EUR 389

Gtpbp8 sgRNA CRISPR Lentivector set (Rat)

K7385901 3 x 1.0 ug
EUR 406.8

Lenti ORF particles, Gtpbp8 (GFP-tagged ORF) - Rat GTP-binding protein 8 (putative) (Gtpbp8), 200ul, >10^7 TU/mL

RR210345L4V 200 µl Ask for price

GTPBP8 3'UTR GFP Stable Cell Line

TU059450 1.0 ml
EUR 1672.8

Gtpbp8 3'UTR GFP Stable Cell Line

TU159227 1.0 ml Ask for price

Gtpbp8 3'UTR GFP Stable Cell Line

TU255567 1.0 ml Ask for price

Gtpbp8 sgRNA CRISPR Lentivector set (Mouse)

K4112501 3 x 1.0 ug
EUR 406.8

GTPBP8 sgRNA CRISPR Lentivector set (Human)

K0919501 3 x 1.0 ug
EUR 406.8

GTPBP8 Protein Vector (Rat) (pPM-C-HA)

PV272004 500 ng
EUR 723.6

Lenti ORF particles, Gtpbp8 (Myc-DDK-tagged ORF) - Rat GTP-binding protein 8 (putative) (Gtpbp8), 200ul, >10^7 TU/mL

RR210345L3V 200 µl Ask for price

GTPBP8 Protein Vector (Rat) (pPB-C-His)

PV272002 500 ng
EUR 723.6

GTPBP8 Protein Vector (Rat) (pPB-N-His)

PV272003 500 ng
EUR 723.6

GTPBP8 Protein Vector (Rat) (pPM-C-His)

PV272005 500 ng
EUR 723.6

GTPBP8 Protein Vector (Human) (pPM-C-HA)

PV018987 500 ng
EUR 394.8

GTPBP8 Protein Vector (Human) (pPM-C-HA)

PV018991 500 ng
EUR 394.8

GTPBP8 Protein Vector (Mouse) (pPM-C-HA)

PV187312 500 ng
EUR 723.6

GTPBP8 Protein Vector (Mouse) (pPM-C-HA)

PV187316 500 ng
EUR 723.6

ARP95882_P050-25UL - GTPBP8 Antibody - middle region

ARP95882_P050-25UL 25ul
EUR 99

OAAB00610-400UL - GTPBP8 Antibody - middle region

OAAB00610-400UL 400ul
EUR 389

GTPBP8 Protein Vector (Human) (pPB-C-His)

PV018985 500 ng
EUR 394.8

GTPBP8 Protein Vector (Human) (pPB-N-His)

PV018986 500 ng
EUR 394.8

GTPBP8 Protein Vector (Human) (pPM-C-His)

PV018988 500 ng
EUR 394.8

GTPBP8 Protein Vector (Human) (pPB-C-His)

PV018989 500 ng
EUR 394.8

GTPBP8 Protein Vector (Human) (pPB-N-His)

PV018990 500 ng
EUR 394.8

GTPBP8 Protein Vector (Human) (pPM-C-His)

PV018992 500 ng
EUR 394.8

GTPBP8 Protein Vector (Mouse) (pPB-C-His)

PV187310 500 ng
EUR 723.6

GTPBP8 Protein Vector (Mouse) (pPB-N-His)

PV187311 500 ng
EUR 723.6

GTPBP8 Protein Vector (Mouse) (pPM-C-His)

PV187313 500 ng
EUR 723.6

GTPBP8 Protein Vector (Mouse) (pPB-C-His)

PV187314 500 ng
EUR 723.6

GTPBP8 Protein Vector (Mouse) (pPB-N-His)

PV187315 500 ng
EUR 723.6

GTPBP8 Protein Vector (Mouse) (pPM-C-His)

PV187317 500 ng
EUR 723.6

Gtpbp8 3'UTR Luciferase Stable Cell Line

TU109227 1.0 ml Ask for price

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